Fig. 1

Differential expression analysis between contrasting SSC samples from the 'O × N' population. A Differentially expressed genes between LowSSC and HighSSC samples are represented in a green-yellow color scale heatmap (FDR < 0.05). Data was scaled using a z-score scaling method, dividing the mean value of each gene by the standard deviation. Each column represents the average expression of three replicates for each selected individual. B Partial least square discriminant analysis (PLS-DA) using normalized counts of each sequenced library. Each symbol represents the three replicates of each selected individual. Yellow and green symbols represent LowSSC and HighSSC samples. C Volcano plot with differentially expressed genes comparing LowSSC and HighSSC samples. Candidate genes for each group were selected with a p-value < 0.01 and |log₂FC| > 1.0. D Gene ontology term enrichment analysis with genes overexpressed in LowSSC and HighSSC samples. The scale color represents the adjusted p-value, and the point size represents the gene ratio