Table 1 Primer pairs used for cloning and detection of mutations in Hd1
Primer name | Sequence (5′-3′) | Annealing temp. (oC) | Product Size (bp) |
|---|---|---|---|
Primer pairs for cloning of Hd1 genomic fragment | |||
Hd1_FP1 | AAAGCAAAGATGAACAGAGGTG | 57.0 | 700 |
Hd1_RP1 | AGAGATTTTACAGGGATCAATAG | ||
Hd1_FP2 | GAGATGTCCATTGAATTGTTTAG | 60.4 | 701 |
Hd1_RP2 | CCTTGCTTGTGGTAGTAGTAG | ||
Hd1_FP3 | GAGGACAAACACAATAGCTTG | 57.0 | 938 |
Hd1_RP3 | AGCTAGTAATAGATGAACTCAC | ||
Hd1_FP4 | ATAGTGGTTATGGAGTTGTG | 62.0 | 944 |
Hd1_RP4 | GTTTCATAACGTATTGTCTTCTC | ||
Hd1_FP5 | CTGGAGCAATCAATCTCTTC | 57.0 | 1095 |
Hd1_RP5 | CTCATGGTTTAGTGAGAAGATAG | ||
Allele-specific primer pairs for the nonsynonymous frameshift mutation in Hd1 | |||
Hd1_Wild_FP | AGGGAGGCCAGGGTGCTCATG | 66.5 | 200 |
Hd1_common RP | CTACTGTCAGATAGAGCTGCAGTGGAGAACATC | ||
Hd1_Mutant_FP | GGGAGGCCAGGGTGCTCCGT | 66.0 | 200 |
Hd1_common RP | CTACTGTCAGATAGAGCTGCAGTGGAGAACATC | ||