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Fig. 1 | Biological Research

Fig. 1

From: GLUT1 and prorenin receptor mediate differential regulation of TGF-β and CTGF in renal inner medullary collecting duct cells during high glucose conditions

Fig. 1

Characterization of mouse inner medullary collecting ducts (IMCD). IMCD cells grown in NG conditions and then treated with HG. A Light field microscopy image shows the characteristic shape and phenotype of the primary cultured IMCD cells (Scale bar: 100 μm). The zoom in image shows the cuboidal characteristic shape of primary cultures of IMCD cells after 6 d. B IMCD were characterized by the expression of collecting duct marker AQP-2 (green) in the plasma membrane (arrows) and cytoplasm (asterisk) of the principal cells. C IMCD cells were stained with PRR (green) showing some PRR-positive cells as well as negative cells (wide arrows) which are mainly principal cells. Scale bar for B and C: 20 μm. Co-localization experiments further demonstrate no colocalization of AQP-2 with GLUT1 (D) or PRR (E). However, GLUT1 colocalized with PRR (F). Staining of nucleus with DAPI. Scale bar for D, E and F: 20 μm. Immunofluorescence and phase contrast images were obtained using a Nikon Eclipse-50i immunofluorescence microscope (Nikon Eclipse-50i, Japan) and were digitalized using the NIS-Elements version 4.0 from Nikon

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Biological Research

ISSN: 0717-6287

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