Fig. 2

GLUT1 expression following 48 h conditioning with NG, HG, or HG + GLUT1 inhibitor BAY 876 pretreatment. A mRNA levels of GLUT1 under treatment with HG alone were augmented almost twice that of controls. GLUT1 inhibition by BAY 876 (2 nM) for 48 h prevented the upregulation of GLUT1 (*p < 0.05, n = 5). B Similar results were observed when analyzing protein levels (*p < 0.05, n = 3). C Four representative immunofluorescence fields showing that GLUT1 abundance (red) was augmented after 48 h HG treatment, while HG + BAY 876 prevented this effect (nuclei in blue). Scale bar represents 20 µm