Fig. 3

HG conditions stimulate prorenin and renin release to the cell culture media, PRR upregulation, and activation of MAPK pathway; GLUT1 inhibition prevented these increases. A Prorenin and renin band detected by immunoblot in concentrated cell culture media. Intensity of prorenin-renin bands (together) was augmented in cell culture media in HG conditions but was prevented in cells pretreated with BAY 876. 40 μg total protein were loaded in each lane. B PRR was augmented in cell lysates of IMCD cells under both HG and HG + BAY 876 conditions, however, incubation with BAY 876 mitigated the extent of the response. C Phospho-ERK1/2 versus total ERK1/2 ratio was augmented after 48 h of HG; BAY 876 pretreatment impaired this effect. *p < 0.05 vs NG; **p < 0.01 vs NG