Fig. 4

IMCD cell protein and mRNA levels of TGF-β and CTGF following high glucose treatment and GLUT1 blockade. Immunoblot of IMCD cells measuring TGF-β and CTGF protein expression under the conditions of NG, HG, and HG plus the GLUT1 inhibitor BAY 876 for 48 h (HG + BAY) is shown along with fold change of treatment compared to NG expressed as the ratio of protein to housekeeping gene β-actin. The bottom-most plot shows the mRNA abundance as fold change of treatment compared to NG. mRNA levels were normalized using housekeeping gene β -actin. No effect was observed in NG + BAY 876 (not shown). *p < 0.05 versus NG, n = 3–4