Fig. 3

Ezh1/2 function deficiency suppresses the phosphorylation of ATM in mouse fetal mice. (A) Representative images of IF staining of ATM (red) and DDX4 (green) in fetal ovaries treated with EZH1/2 inhibitor (UNC1999). The nucleus was stained with DAPI (blue). Scale bar: 50 μm, enlarged scale bar: 10 μm; (B-D) The level of p-ATM in fetal ovaries treated with UNC1999 (B), GSK343(C), and lentivirus (D, dKO). Green is the positive color for p-ATM. DDX4 is stained red. The nucleus was stained with DAPI (blue). Scale bar: 50 μm, enlarged scale bar: 10 μm; (E) Quantitative analysis of ATM fluorescence intensity in A. (F-H) Quantitative analysis of p-ATM fluorescence intensity in B-D. (I) The level of p-ATM in WT or dKO ovaries checked by WB. GAPDH was used as an internal control. The ratio of p-ATM to ATM protein is shown in the right figure. Every group had 20 ovaries. The number of images assessed in a single experiment was at least 3. The experiments were repeated at least 3 times (N = 3). The data were presented as mean ± S.D. *P < 0.05, **P < 0.01 (t-test)