Fig. 2
From: Lack of canonical activities of connexins in highly aggressive human prostate cancer cells
Relative amount of connexin proteins in human prostate cancer cell lines. The relative amount of Cx26 (A), Cx32 (C), Cx43 (E), Cx37 (G), Cx40 (I) and Cx45 (K) protein was analyzed in LNCaP, LNCaP-C4-2, DU-145 and PC-3 PCa cell lines using western blot analysis. β-actin (Actin) was used as a loading control. MDA-MB-231 cell line protein extract was utilized for positive controls. The densitometric analyses of the western blots for Cx26 (B), Cx32 (D) and Cx43 (F) Cx37 (H), Cx40 (J) and Cx45 (L) were carried out using ImageJ software. One-way ANOVA followed by Tukey’s tests were utilized for statistical analyses. * = P < 0.05, ** = P < 0.001. Each bar represents the mean ± SD of 3 independent experiments