Fig. 5
From: Lack of canonical activities of connexins in highly aggressive human prostate cancer cells
Dye uptake in human prostate cancer cell lines. DAPI (5 µM) uptake was measured every 15 s in LNCaP, LNCaP C4-2, Du-145 and PC-3 cell line cultures. Fluorescence intensity due to DAPI uptake was expressed as arbitrary units (AU). After 5 min, PCa cells were exposed to a medium without divalent cations to stimulate the opening of hemichannels. At the end of the experiment, La+ 3 (200 µM) was added to the recording solution to block the hemichannels. (A-D) DAPI uptake rate in PCa cell lines under basal conditions, under hemichannel stimulation and blockade. (D) Relative DAPI uptake (uptake rate under stimulus / basal uptake rate) in PCa cell lines. In each experiment the fluorescence intensity was measured over time in at least 20 cells. Each bar represents the average ± SD of 3 independent experiments, each one performed in triplicate. One-way ANOVA followed by Tukey’s tests were utilized for statistical analyses. * = P < 0.05, ** = P < 0.01, *** = P < 0.001