Fig. 1
From: Study on the therapeutic potential of induced neural stem cells for Alzheimer's disease in mice
Generation of iNSCs from mouse fibroblasts. A Immunofluorescence microscopy images of using Vimentin/α-SMA. B The process of cellular transformation: the morphology of the MEFs was changed at 3 days after transfection in the SM, S, and M group; by 7 days after transduction, there were generated neurospheres similar to wild-type NSCs. C Quantitative plot of the number of neurospheres. D qRT-PCR reveals that iNSCs express typical NSC markers in D but do not express pluripotency-related genes F. E a1-2: the p1 generation of SM group; b1-2: the p3 generation of SM group; The iNSCs expressed NSCs makers, including Sox2(c1-4) and Nestin (d1-4). G Expression levels of Sox2 and Nestin in different cells by WB