Fig. 1

Location of Protein Palmitoylation in Mouse Sperm. (A) In situ protein palmitoylation in mouse sperm, with palmitoylation depicted in green (Alexa Fluor 488) and nuclei depicted in blue (DAPI). Palmitoylation (Palm) was labeled using click chemistry with azide; the negative control (Control) lacked azide. (B) Fluorescence intensity highlights the distinction between protein palmitoylation and the negative control. The fluorescent intensity was processed and analyzed with ImageJ. Data are presented as means ± SEM (n, number of analyzed images from three distinct samples, one mouse per individual sample). A P-value < 0.05 denotes statistical significance. (C) Schematic illustrating the procedure of the click-IT method