Fig. 5
Effect of 2BP on Protein Palmitoylation in Sperm. (A) Effect of 2BP on protein palmitoylation in sperm. Protein palmitoylation in sperm treated with 100 µM 2BP at different time points was detected using ABE. The loading controls were confirmed by Western blotting. Bands in lanes with NH2OH represent palmitoylated proteins. (B) Quantification of protein palmitoylation levels is indicated by the arrows in (A). Data are presented as means ± SEM (n = 3 repeats, pooled samples from 12 mice per repeat), with the DMSO group as the vehicle control. A P-value < 0.05 denotes statistical significance