Fig. 8
Regulation of Palmitoylated HSP90 by PKA/PKC Inhibitors and Calcium Chelators. (A) Effect of H89 and CC on palmitoylated HSP90. The bands indicated by the arrow are palmitoylated HSP90 with NH2OH, with quantification shown in (C). (B) Effect of calcium chelators on palmitoylated HSP90, with quantification displayed in (D). (E) Procedures for detecting palmitoylated HSP90: palmitoylated HSP90 was detected using an anti-HSP90 primary antibody after streptavidin-conjugated beads bound to biotinylated proteins were treated using the ABE method and separated using SDS-PAGE. Gray intensities of palmitoylated HSP90 were quantified using ImageJ software and normalized to the total amount of loaded protein. Data are presented as means ± SEM (n = 3 or 4 repeats, pooled samples from 12 mice per repeat), with the DMSO group serving as the vehicle control. A P-value < 0.05 denotes statistical significance