Fig. 3

CD45-mediated dephosphorylation of various kinases in aged MSCs and its reversal by the PTP inhibitor. The figure shows the expression of phosphorylated and native forms of p38 (A), ERK1/2 (B), SAPK/JNK (C), Src kinase (D), GSK3β (E) and Beta-actin and their corresponding densitometry graphs [Aa, B(a–b), C(a–b), Da, Ea.] in young, aged, and PTP inhibitor-treated aged MSCs. The band intensities of phosphorylated proteins were normalized with respect to their native forms, and the fold change of the ratio of phosphorylated proteins versus native proteins was plotted. MSCs mesenchymal stem cells, PTP protein tyrosine phosphatase, Young young MSCs, Aged aged MSCs, A + PTP− aged MSCs treated with PTP inhibitor