Fig. 4

Pharmacological inhibition of p38, MAPK and SRC kinases in young MSCs imposes an aged-like gene signature on them. The figure shows qRT-PCR analyses for Runx-2 (a), Alp-2 (b), Rankl (c), ostf1 (d), and pparg (e) gene expression at mRNA level in young MSCs treated with a p38 inhibitor, MAPK inhibitor, a combined treatment of p38 and MAPK inhibitors and PP1 separately. Data represent the relative expression of transcripts normalized relative to GAPDH and expressed as the Mean ± SEM for three biologically independent experiments (n = 3). For statistical analysis, between different groups, one-way ANOVA followed by Bonferroni post hoc test in Graphpad Prism 6 version 6.0 (GraphPad Software Inc, La Jolla, CA) was used. Error bars in the graph indicate the standard deviation (SD). All the data are represented as mean ± SD. *p < 0.05; **p < 0.01; ***p < 0.001, ****p < 0.0001; ns not significant