Fig. 4

CHIP targets HG-induced p53 for ubiquitin-mediated proteasomal degradation in WJMSCs. (A-C) WJMSCs transfected with HA-CHIP plasmid in the presence of MG-132 for 6 h were challenged with HG for 3 d. Total cellular lysate immunoprecipitated with the anti-HA, anti-P-p53, and anti-CHIP antibodies were further immunoblotted with anti-HA, anti-P-p53, and anti-ubiquitin antibody. (D and E) WJMSCs transfected with HA-vector, HA-CHIP, and CHIP mutants (K30A and H260Q) were challenged with HG in the absence and presence of MG-132 for 6 h. Total cellular lysate immunoprecipitated with the anti-HA and anti-p53 antibody was subsequently immunoblotted with the anti-HA, anti-P-p53, and anti-ubiquitin antibody. β-actin employed as internal loading control and *p < 0.05, and **p < 0.01 indicates the significance (n = 3 per group)