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Fig. 5 | Biological Research

Fig. 5

From: Chaperone-assisted E3 ligase-engineered mesenchymal stem cells target hyperglycemia-induced p53 for ubiquitination and proteasomal degradation ameliorates self-renewal

Fig. 5

Chaperone system is involved in proteasomal degradation of HG-induced p53 in WJMSCs. (A) WJMSCs transfected with either HA-CHIP, shCHIP or treated with GA (HSP90 inhibitor) were challenged with the HG and total cellular lysate was analyzed to measure the protein expression of CHIP, HA and phosphorylated p53 using western blot analysis. (B) WJMSCs transfected with HA-CHIP, shCHIP, and siHSP70 were incubated with HG and immunoblotted to measure the expression level of CHIP, HA-tagged CHIP, and phosphorylated p53. (C) Graphical representation illustrating that CHIP targets HG-induced p53 for ubiquitination-mediated proteasomal degradation ameliorates self-renewal factors. β-actin employed as internal loading control and *p < 0.05, and **p < 0.01 indicates the significance (n = 3 per group)

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Biological Research

ISSN: 0717-6287

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