Fig. 1
From: Ptprd deficiency promotes tau hyperphosphorylation and impairs cognitive function in aged mice
Ptprd absence causes tau hyperphosphorylation in the mouse brain. Western blot analysis of cortical (A, C) and hippocampal lysates (E, G) from 6-month and 18-month-old Ptprd WT and Ptprd KO mice, probed with AT8, AT100, AT180, pSer396, pSer403, or pSer422 antibodies to detect phosphorylated tau, and with an antibody against total tau (Tau5) and HSP90 as a loading control. Quantification by scanning densitometry of the relative level of tau phosphorylation/total tau in Western blot analysis was performed in cortical lysates at 6 months (B) and 18-months-old mice (D) and in hippocampal lysates at 6 months (F) and 18-months-old (H). *p < 0.05; n = 4–12