Fig. 2

Ptprd loss causes Abl1 kinase activation in the mouse hippocampus. Western blot analysis of cortical (A) and hippocampal lysates (C) from 18-month-old Ptprd WT and Ptprd KO mice, probed with antibodies for Abl1 phosphorylated at tyrosine 412, Gsk3β phosphorylated at serine 9, or Cdk5 phosphorylated at tyrosine 15. To assess the relative levels of kinase activation, the same lysates were analyzed on a western blot using antibodies that recognized total Abl1, Gsk3β, or Cdk5. The lysates were also analyzed for levels of HSP90 as a loading control. Quantification of the relative levels of Abl1, Gsk3β, and Cdk5 phosphorylation was assessed by densitometry of cortical (B) and hippocampal western blots (D). *p < 0.05; n = 4–7