Fig. 4

Ptprd KO mice show increased neuroinflammation in the entorhinal cortex and hippocampus. Iba1-positive cells (green) were immunolabeled in the brain entorhinal cortex (A) and hippocampus (E) of Ptprd WT and Ptprd KO mice at 18 months of age, and sections were counterstained with Hoechst 33,258 (blue). The total number of microglia per mm² was quantified in 6- and 18-month-old brain entorhinal cortex (B) and hippocampus (F). The mean microglial soma size was measured in the cortex (C) and hippocampus (G) at 18 months. IL1β mRNA expression was measured by qPCR in cortical (D) and hippocampal samples (H) from 18-month-old mice. Scale bar = 200 μm. *p < 0.05, **p < 0.01, ***p < 0.001; n = 4-6