Fig. 4
From: NAMPT regulates mitochondria and oxidative stress level for mouse early embryo development
Loss of NAMPT activity induces oxidative stress in mouse embryos. (A) Typical images for the 2-cell embryo staining with MMP after 50 µM dose of FK866 treatment. Red, MMP; blue, DNA. Bar = 20 μm. (B) The fluorescence intensity data for the MMP showed the significantly decrease after inhibition of NAMPT activity. ***, P < 0.001. (C) Typical images for the 2-cell embryo staining with ROS after 50 µM dose of FK866 treatment. Green, ROS. Bar = 100 μm. (D) The percentage of ROS positive embryos increased in the FK866 treatment groups compared with the control. ***, P < 0.01. (E) The fluorescence intensity data for the ROS showed the significantly increase after inhibition of NAMPT activity. *, P < 0.05