Fig. 2

Kit ^D17/+ mice presented lower sperm quality. (A) H&E staining of mouse epididymal sections. Scale bar = 50 μm (scale bar in magnified image = 30 μm), n = 3. (B) Mouse sperm number and sperm viability assay using the CASA system. The trajectories of the sperm were analyzed. Blue indicates fast trajectories, green indicates moderate-speed trajectories, and yellow indicates slow trajectories. Ten 1.5 s videos were recorded for each mouse. (C-M) Statistical graphs of sperm number, sperm viability, distance average path (DAP), average path velocity (VAP), distance curvilinear (DCL), distance straight line (DSL), straight line velocity (VSL), curvilinear velocity (VCL), amplitude of lateral head displacement (ALH), beat-cross frequency (BCF), and wobble (ratio of VSL/VCL). Student’s t test; **P < 0.01, *P < 0.05. (N) Litter sizes of (♂Kit ^+/+ × ♀Kit ^+/+), (♂ Kit ^D17/+ × ♀ Kit ^+/+), and (♂ Kit ^+/+ × ♀ Kit ^D17/+) mice, n ≥ 3. Student’s t test; **P < 0.01, *P < 0.05