Fig. 3

Deleting exon 17 of Kit impaired spermatogonia differentiation. Immunostaining of Pgp9.5⁺ (A), Sox9⁺ (B), Dazl⁺ (C), Ddx4⁺ (D) and Sycp3⁺ (E) in testicular tissue sections from Kit ^D17/+ and Kit ^+/+ mice. Scale bar = 50 μm. (F-I) Quantitative analysis of the proportions of Pgp9.5⁺ (A), Sox9⁺ (B), Dazl⁺ (C), Ddx4⁺ (D) and Sycp3⁺ (E) cells in testicular tissue sections from Kit ^D17/+ and Kit ^+/+ mice. The data are presented as the mean ± SEM of Kit ^D17/+ and Kit ^+/+ males, with 50 tubule cross-sections analyzed per individual, n = 3. Student’s t test; **P < 0.01, *P < 0.05