Fig. 5

Gene ontology analysis and RNA-seq validation by RT‒qPCR analysis. (A and C) Gene Ontology enrichment of biological processes associated with the down- and upregulated DEGs between testicular tissues of Kit ^+/+ and Kit ^D17/+ mice at 5 weeks of age. (B and D) Gene Ontology enrichment of biological processes associated with the down- and upregulated DEGs between testicular tissues of 7-week-old Kit^+/+ and Kit ^D17/+ mice, n = 2 or 3. (E) Heatmap of spermatogenesis-related gene expression. (F) Heatmap of meiosis-related gene expression. (G-L) RT‒qPCR validation of DEGs, n = 3. Student’s t test; **P < 0.01, *P < 0.05