Fig. 6
KEGG enrichment analysis and western blotting analysis. (A) Common signaling pathways in the top 30 enriched KEGG pathways of DEGs between testicular tissues of 5-week-old and 7-week-old Kit+/+ and KitD17/+ mice. (B) Protein expression assay for KIT, KIT (P), RAF, RAF (P), MEK1/2, MEK1/2 (P), ERK1/2, and ERK1/2 (P) in Kit D17/+ and Kit +/+ testis tissue. β-actin and GAPDH served as loading controls, n = 3. (C-J) Quantification of protein expression for KIT, KIT (P), ERK1/2, ERK1/2 (P), RAF, RAF (P), MEK1/2, and MEK12 (P) based on the intensity of the blotting bands. The data are shown as the mean (± SEM) of three independent experiments. Student’s t test; **P < 0.01, *P < 0.05